Production and Screening of High Yield Avermectin B1b Mutant of Streptomyces avermitilis 41445 Through Mutagenesis
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منابع مشابه
Production and Screening of High Yield Avermectin B1b Mutant of Streptomyces avermitilis 41445 Through Mutagenesis
BACKGROUND Secondary metabolite production from wild strains is very low for economical purpose therefore certain strain improvement strategies are required to achieve hundred times greater yield of metabolites. Most important strain improvement techniques include physical and chemical mutagenesis. Broad spectrum mutagenesis through UV irradiation is the most important and convenient physical m...
متن کاملproduction and screening of high yield avermectin b1b mutant of streptomyces avermitilis 41445 through mutagenesis
conclusions: the hereditary stability analysis of the uv mentioning 45 minutes revealed the uv exposure time for mutants and 3 represented the colony taken from the plate irradiated for 45 minutes mutant showed that the production of avermectin b1b remained constant and no reverse mutation occurred after 15 generations. results: avermectin b1b-hyper-producing mutant, produced using these three ...
متن کاملproduction of avermectin b1b from streptomyces avermitilis 41445 by batch submerged fermentation
results however the maximum production of avermectin b1b (17 mg/l) was obtained by using sm2 growth medium containing soluble corn starch, yeast extract, kcl, caco3 and mgso4 which was detected qualitatively by using tlc and quantitatively by hplc. conclusions maximum production was observed with initial medium ph of 7, 10% inoculum size with incubation temperature of 31°c for 10 days of fermen...
متن کاملControl of avermectin biosynthesis in Streptomyces avermitilis for the selective production of a useful component.
I. Biosynthesis of Avermectins A. Incorporation of Labeled Precursors B. Biosynthetically Blocked Mutants and Characteristics of Each Step in Avermectin Biosynthetic Pathway 1. aveA {aveAI and aveAIh step for polyketide synthase) 2. aveB (step for glycosylation) 3. aveC (step for C22~23-dehydration) 4. aveD (step for C5 O-methylation) 5. aveE (step for furan ring formation at C6 to C8a) 6. aveF...
متن کاملSignificance of Heavy-Ion Beam Irradiation-Induced Avermectin B1a Production by Engineered Streptomyces avermitilis
Heavy-ion irradiation technology has advantages over traditional methods of mutagenesis. Heavy-ion irradiation improves the mutation rate, broadens the mutation spectrum, and shortens the breeding cycle. However, few data are currently available regarding its effect on Streptomyces avermitilis morphology and productivity. In this study, the influence of heavy-ion irradiation on S. avermitilis w...
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ژورنال
عنوان ژورنال: Jundishapur Journal of Microbiology
سال: 2014
ISSN: 2008-3645,2008-4161
DOI: 10.5812/jjm.8626